Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Fumarate hydratase antibody [GT7612] diluted at 1:500.Application
Fumarate hydratase antibody [GT7612] detects Fumarate hydratase protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 5 min. Green: Fumarate hydratase protein stained by Fumarate hydratase antibody [GT7612] diluted at 1:500. Blue: Hoechst 33342 staining.Application
Fumarate hydratase antibody [GT7612] detects Fumarate hydratase protein by Western blot analysis. A. 30 µg 293T B. 30 µg A431 C. 30 µg HeLa D. 30 µg HepG2 10 % SDS-PAGE Fumarate hydratase antibody [GT7612] dilution: 1:500Application
Fumarate hydratase antibody [GT7612] detects Fumarate hydratase protein by Western blot analysis. A. 50 µg mouse liver lysate/extract 10 % SDS-PAGE Fumarate hydratase antibody [GT7612] dilution: 1:1000Application
Fumarate hydratase antibody [GT7612] detects Fumarate hydratase protein by Western blot analysis. A. 50 µg rat liver lysate/extract 10 % SDS-PAGE Fumarate hydratase antibody [GT7612] dilution: 1:1000| Product Name | Fumarate hydratase antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [GT7612] to Fumarate hydratase (fumarate hydratase)The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Fumarate hydratase. The exact sequence is proprietary. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG3 |
| Clone Number | GT7612 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Zebrafish, Pig, Chicken, Sheep, Chimpanzee, BovineZebrafish (90%), Pig (98%), Chicken (91%), Sheep (99%), Chimpanzee (99%), Bovine (98%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | fumarate hydratase FH HLRCC MCL MCUL1 LRCC |
|---|---|
| Target | Fumarate hydratase |
| Cellular Localization | Mitochondrion , Cytoplasm |
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Fumarate hydratase antibody [GT7612] diluted at 1:500.
Application
Fumarate hydratase antibody [GT7612] detects Fumarate hydratase protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 5 min. Green: Fumarate hydratase protein stained by Fumarate hydratase antibody [GT7612] diluted at 1:500. Blue: Hoechst 33342 staining.
Application
Fumarate hydratase antibody [GT7612] detects Fumarate hydratase protein by Western blot analysis. A. 30 µg 293T B. 30 µg A431 C. 30 µg HeLa D. 30 µg HepG2 10 % SDS-PAGE Fumarate hydratase antibody [GT7612] dilution: 1:500
Application
Fumarate hydratase antibody [GT7612] detects Fumarate hydratase protein by Western blot analysis. A. 50 µg mouse liver lysate/extract 10 % SDS-PAGE Fumarate hydratase antibody [GT7612] dilution: 1:1000
Application
Fumarate hydratase antibody [GT7612] detects Fumarate hydratase protein by Western blot analysis. A. 50 µg rat liver lysate/extract 10 % SDS-PAGE Fumarate hydratase antibody [GT7612] dilution: 1:1000| Positive Control | 293T , A431 , HeLa , HepG2 , mouse liver , rat liver |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
Data sheet for OM253709