Application
Immunofluorescence staining of Fyn in human HeLa cell line using anti-Fyn ( green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).Application
Immunoprecipitation of Fyn from the lysate of T cells isolated from fresh buffy coats. Western blot was immunostained with anti-Fyn . Lane 1: original lysate of T cells Lane 2-3: Immunoprecipitated material eluted from affinity sorbent (FYN-01 coupled to Sepharose beads). Lanes differ in amount of T cell lysate loaded on the immunosorbent.Application
Immunofluorescence staining of Fyn in murine transformed fibroblasts using anti-Fyn ( red). Actin cytoskeleton was decorated by phalloidin (green) and cell nuclei stained with DAPI (blue).Application
Immunofluorescence staining of Fyn in human primary fibroblasts using anti-Fyn ( green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).| Product Name | FYN antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [FYN - 01] to FynThis gene is a member of the protein-tyrosine kinase oncogene family. It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Alternatively spliced transcript variants encoding distinct isoforms exist. |
| Immunogen | Recombinant fragment, corresponding to amino acids 7-176 of Human Fyn. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | FYN-01 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIPWB |
| ICC: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: 59 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 137025 P06241 MGC45350 SYN SLK 2534 FYN |
|---|---|
| Target | FYN |
| Tissue Specificity | The antibody reacts with Fyn, a non receptor protein tyrosine kinase (59 kDa),which belongs to the Src family of cytoplasmic tyrosine kinases. |
| Cellular Localization | Cytoplasmic |
Application
Immunofluorescence staining of Fyn in human HeLa cell line using anti-Fyn ( green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).
Application
Immunoprecipitation of Fyn from the lysate of T cells isolated from fresh buffy coats. Western blot was immunostained with anti-Fyn . Lane 1: original lysate of T cells Lane 2-3: Immunoprecipitated material eluted from affinity sorbent (FYN-01 coupled to Sepharose beads). Lanes differ in amount of T cell lysate loaded on the immunosorbent.
Application
Immunofluorescence staining of Fyn in murine transformed fibroblasts using anti-Fyn ( red). Actin cytoskeleton was decorated by phalloidin (green) and cell nuclei stained with DAPI (blue).
Application
Immunofluorescence staining of Fyn in human primary fibroblasts using anti-Fyn ( green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).| Application Notes | ICC: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: 59 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline pH 7.4 containing 15mM sodium azide |
