Application
Sample (30 ug of whole cell lysate) A: Jurkat 10% SDS PAGE diluted at 1:1000Application
FYN antibody [N1C2] detects FYN protein by western blot analysis. Mouse and rat tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with FYN antibody [N1C2] diluted at 1:1000.Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using FYN antibody at 1:500 dilution.| Product Name | FYN antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to FYN (FYN oncogene related to SRC, FGR, YES)This gene is a member of the protein-tyrosine kinase oncogene family. It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Alternatively spliced transcript variants encoding distinct isoforms exist. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human FYN. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Pig, Chicken, BovineXenopus laevis (94%), Pig (95%), Chicken (100%), Bovine (95%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MGC45350 antibody SLK antibody SYN antibody FYN antibody OKT3-induced calcium influx regulator antibody src-like kinase antibody proto-oncogene Syn antibody proto-oncogene c-Fyn antibody tyrosine kinase p59fyn(T) antibody c-syn protooncogene antib |
|---|---|
| Molecular Weight(MW) | 61 kDa (note) (2) |
| Cellular Localization | Cell membrane |
Application
Sample (30 ug of whole cell lysate) A: Jurkat 10% SDS PAGE diluted at 1:1000
Application
FYN antibody [N1C2] detects FYN protein by western blot analysis. Mouse and rat tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with FYN antibody [N1C2] diluted at 1:1000.
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using FYN antibody at 1:500 dilution.| Positive Control | Jurkat , Mouse brain , Rat brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
