Rabbit polyclonal to G protein alpha 16G alpha 16 is the only hetero- trimeric G proteins with a restricted expression pattern in hematopoietic cells. Differentiation of promyelocytic cells leads to decreased expression of G alpha 16. This G protein is known to couple a large number of G protein-coupled receptors to PLC-b and can lead to production of the secondary messengers diacylglycerol and inositol phosphates. Targeted deletion of G alpha 15, a mouse orthologue of human G alpha 16, causes reduced C5a receptor signaling. G alpha 16 serves as a marker, in addition to CD34, for hematopoietic progenitor cells.
Immunogen
Recombinant protein -amino acids 237-373 of human G protein alpha 16
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
IPWB
For WB: Use at a dilution of 1:4,500 - 1:5,500. The use of this product can also be used for IP but it is not yet reported. Optimal dilutions/concentrations should be determined by the researcher.:
Species Reactivity
Human
Concentration
1mg/ml (Please refer to the vial label for the specific concentration)
Purification
Affinity purified
Target Information
Gene Synonyms
G protein alpha16 GNA16
Target
G protein alpha 16
Application
Application
Ga1 6 detected by Western blot in transfected HEK293 cells (right lane)
Application Notes
For WB: Use at a dilution of 1:4,500 - 1:5,500. The use of this product can also be used for IP but it is not yet reported. Optimal dilutions/concentrations should be determined by the researcher.:
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.