Application
Sample(30 ug whole cell lysate) A: A431 B: HeLa S3 12% SDS PAGE diluted at 1:500Application
G protein alpha Inhibitor 1 antibody detects GNAI1 protein at cytoplasm on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. G protein alpha Inhibitor 1 antibody dilution: 1:500.| Product Name | G protein alpha Inhibitor 1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to G protein alpha Inhibitor 1 (guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1)Guanine nucleotide-binding proteins (G proteins) form a large family of signal-transducing molecules. They are found as heterotrimers made up of alpha, beta, and gamma subunits. Members of the G protein family have been characterized most extensively on the basis of the alpha subunit, which binds guanine nucleotide, is capable of hydrolyzing GTP, and interacts with specific receptor and effector molecules. The G protein family includes Gs (MIM 139320) and Gi, the stimulatory and inhibitory GTP-binding regulators of adenylate cyclase; Go, a protein abundant in brain (GNAO1; MIM 139311); and transducin-1 (GNAT1; MIM 139330) and transducin-2 (GNAT2; MIM 139340), proteins involved in phototransduction in retinal rods and cones, respectively (Sullivan et al., 1986 [PubMed 3092218]; Bray et al., 1987 [PubMed 3110783]). Suki et al. (1987) [PubMed 2440724] concluded that the human genome contains at least 3 nonallelic genes for alpha-i-type subunits of G protein; see, e.g, GNAI2 (MIM 139360), GNAI3 (MIM 139370), and GNAIH (MIM 139180).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human G protein alpha Inhibitor 1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Xenopus laevis, Pig, Chicken, Bovine, Guinea pigMouse (100%), Rat (99%), Zebrafish (96%), Xenopus laevis (97%), Pig (90%), Chicken (97%), Bovine (100%), Guinea pig (99%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | Gi antibody GNAI1 antibody Gi1 protein alpha subunit antibody adenylate cyclase-inhibiting G alpha protein antibody guanine nucleotide-binding protein G(i) subunit alpha-1 antibody guanine nucleotide binding protein (G protein) alpha inhibiting activ |
|---|---|
| Molecular Weight(MW) | 40 kDa (note) (2) |
Application
Sample(30 ug whole cell lysate) A: A431 B: HeLa S3 12% SDS PAGE diluted at 1:500
Application
G protein alpha Inhibitor 1 antibody detects GNAI1 protein at cytoplasm on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. G protein alpha Inhibitor 1 antibody dilution: 1:500.| Positive Control | A431 , HeLaS3 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
