Application
Western blot analysis of extracts from 293 cells untreated or treated with starvation using G3BP-1 (Phospho-Ser232) AntibodyApplication
Western blot analysis of extracts from 293 cells untreated or treated with starvation using G3BP-1 (Phospho-Ser232) Antibody .Application
Immunofluorescence staining of methanol-fixed Hela cells using G3BP-1 (Phospho-Ser232) Antibody .Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using G3BP-1 (Phospho-Ser232) Antibody (left) or the same antibody preincubated with blocking peptide (right).| Product Name | G3BP-1 (phospho Ser232) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to G3BP-1 (Phospho Ser232)May be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium-dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA. |
| Immunogen | Peptide sequence around phosphorylation site of serine 232 (S-S-S(p)-P-A) derived from Human G3BP-1. |
| Modification | p-Ser232 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | G3BP HDH-VIII MGC111040 G3BP1 RasGAP-associated endoribonuclease G3BP GAP SH3 domain-binding protein 1 G3BP-1 ras GTPase-activating protein-binding protein 1 Ras-GTPase-activating protein SH3-domain-binding protein GAP binding protein ATP-depende |
|---|---|
| Target | G3BP-1 (Phospho Ser232) |
| Molecular Weight(MW) | 60 (note) |
Application
Western blot analysis of extracts from 293 cells untreated or treated with starvation using G3BP-1 (Phospho-Ser232) Antibody
Application
Western blot analysis of extracts from 293 cells untreated or treated with starvation using G3BP-1 (Phospho-Ser232) Antibody .
Application
Immunofluorescence staining of methanol-fixed Hela cells using G3BP-1 (Phospho-Ser232) Antibody .
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using G3BP-1 (Phospho-Ser232) Antibody (left) or the same antibody preincubated with blocking peptide (right).| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
