Application
Sample (30 µg of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 7.5% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded Hela xenograft, using G3BP1 antibody at 1:500 dilution.Application
G3BP1 antibody detects G3BP1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: G3BP1 protein stained by G3BP1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | G3BP1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to G3BP1 (ras GTPase-activating protein-binding protein 1)This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human G3BP1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Pig, Rhesus Monkey, BovineMouse (85%), Rat (86%), Pig (88%), Rhesus Monkey (99%), Bovine (89%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | G3BP antibody HDH-VIII antibody MGC111040 antibody G3BP1 antibody RasGAP-associated endoribonuclease G3BP antibody GAP SH3 domain-binding protein 1 antibody G3BP-1 antibody ras GTPase-activating protein-binding protein 1 antibody Ras-GTPase-activat |
|---|---|
| Molecular Weight(MW) | 52 kDa (note) (2) |
| Cellular Localization | Cytoplasm , cytosol , Cell membrane , Nucleus |
Application
Sample (30 µg of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 7.5% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded Hela xenograft, using G3BP1 antibody at 1:500 dilution.
Application
G3BP1 antibody detects G3BP1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: G3BP1 protein stained by G3BP1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Positive Control | 293T , A431 , HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
