Application
Western blot analysis of G α-15 expression in 3T3 cell lysate.| Product Name | Ga15/16 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Ga15/16Galpha 16 is the only heterotrimeric G protein with a restricted expression pattern in hematopoietic cells. Differentiation of promyelocytic cells leads to decreased expression of Galpha 16. The G protein is known to couple a large number of G protein-coupled receptors to PLC-beta and can lead to production of the secondary messengers diacylglycerol and inositol phosphates. Targeted deletion of Galpha 15, a mouse orthologue of human Galpha 16, causes reduced C5alpha receptor signaling. Galpha 16 serves as a marker, in addition to CD34, for hematopoietic progenitor cells. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western blotting (0.5-4 μg/ml) and in Immunohistochemistry (5-20 μg/ml). However, the optimal conditions should be determined individually. The antibody detects G: |
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| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | G alpha binding protein |
|---|---|
| Target | Ga15/16 |
| Tissue Specificity | This antibody is specific for Ga15/16. |
Application
Western blot analysis of G α-15 expression in 3T3 cell lysate.| Application Notes | Western blotting (0.5-4 μg/ml) and in Immunohistochemistry (5-20 μg/ml). However, the optimal conditions should be determined individually. The antibody detects G: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | The antibody solution should be gently mixed before use. Stored at -20 °C for future use. |
| Storage Buffer | PBS (pH 7.2) containing 30% glycerol, 0.5% BSA, 0.01% thimerosal. |
