Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Gab2 (Phospho-Ser623) antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from 293 cells (Lane 2), HeLa cells (Lane 3) and HepG2 cells (Lane 4), using Gab2 (Phospho-Ser623) Antibody. The lane on the left is treated with antigen-specific peptide.| Product Name | GAB2 (phospho Ser623) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GAB2 (phospho Ser623)This gene is a member of the GRB2-associated binding protein (GAB) gene family. These proteins contain pleckstrin homology (PH) domain, and bind SHP2 tyrosine phosphatase and GRB2 adapter protein. They act as adapters for transmitting various signals in response to stimuli through cytokine and growth factor receptors, and T- and B-cell antigen receptors. The protein encoded by this gene is the principal activator of phosphatidylinositol-3 kinase in response to activation of the high affinity IgE receptor. Two alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq, Nov 2009] |
| Immunogen | Peptide sequence around phosphorylation site of Serine 623(P-S-S(p)-P-S) derived from Human Gab2 . |
| Modification | p-Ser623 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| WB: 1:500 - 1:1000. IHC: 1:50 - 1:100: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | GAB2 GRB2-associated binding protein 2 |
|---|---|
| Target | GAB2 (phospho Ser623) |
| Tissue Specificity | The antibody detects endogenous levels of Gab2 only when phosphorylated at serine 623. |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Gab2 (Phospho-Ser623) antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from 293 cells (Lane 2), HeLa cells (Lane 3) and HepG2 cells (Lane 4), using Gab2 (Phospho-Ser623) Antibody. The lane on the left is treated with antigen-specific peptide.| Application Notes | WB: 1:500 - 1:1000. IHC: 1:50 - 1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20˚C/1 year |
| Storage Buffer | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
