Application
IHC ImageImmunohistochemistry: GABA A Receptor alpha 4 Antibody [] - Immunostaining of rat hippocampus showing labeling of GABAA alpha4 subunitApplication
WB ImageWestern Blot: GABA A Receptor alpha 4 Antibody [] - Western blot of 10 ug of rat brain hippocampus (Hipp) lysate showing immunolabeling of the ~64k a4-subunit of the GABAA -R.Application
WB ImageWestern blot analysis of homogenates from hippocampus using approximately 5-7 ug of tissue per well. Blots were incubated with anti-GABAA alpha4 overnight at 4C then with AP-conjugated goat anti-rabbit IgG.| Product Name | GABA A Receptor alpha 4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to GABA A receptor alpha 4GABA (A) receptors are ligand-gated chloride channels that play a role as inhibitory neurotransmitters. They are known targets for certain classes of environmental and pharmaceutical compounds because a number of drugs interact with binding sites on GABA (A). Some of these drugs include benzodiazepines, anticonvulsants, and anesthetics. GABA (A) Receptors are comprised of subunits and these are further classified into 3 major groups (alpha, beta, and gamma). The subunits determine the pharmacological characteristics. |
| Immunogen | Synthetic peptide from the N-terminus of the rat GABA-A receptor alpha 4 subunit, coupled to KLH. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIPWB |
| We recommend the following starting dilutions: immunoprecipitation 20 ug, Western Blot 1:1,000. Optimal concentration should be determined experimentally by the end user.: |
|
| Species Reactivity | Rat |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Gamma aminobutyric acid receptor alpha 4 subu Gamma aminobutyric acid receptor subunit alph GABA(A) receptor subunit alpha4 GABRA 4 GABR A4 Gamma aminobutyric acid (GABA) A receptor alp Gamma aminobutyric acid A receptor alpha4 a GABA(A) receptor su |
|---|---|
| Target | GABA A receptor alpha 4 |
| Tissue Specificity | NB300-194 reacts with the N-terminus of the 64 kDa alpha 4 subunit of rat brain GABA-A. There is no known reactivity with other GABA subunits. There is no homology with other GABA subunits. |
Application
IHC ImageImmunohistochemistry: GABA A Receptor alpha 4 Antibody [] - Immunostaining of rat hippocampus showing labeling of GABAA alpha4 subunit
Application
WB ImageWestern Blot: GABA A Receptor alpha 4 Antibody [] - Western blot of 10 ug of rat brain hippocampus (Hipp) lysate showing immunolabeling of the ~64k a4-subunit of the GABAA -R.
Application
WB ImageWestern blot analysis of homogenates from hippocampus using approximately 5-7 ug of tissue per well. Blots were incubated with anti-GABAA alpha4 overnight at 4C then with AP-conjugated goat anti-rabbit IgG.| Application Notes | We recommend the following starting dilutions: immunoprecipitation 20 ug, Western Blot 1:1,000. Optimal concentration should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Storage Buffer | 10mM Hepes (pH 7.5), 150 mM NaCl, 100 ug/ml BSA, and 50% glycerol |
