Application
Western Blot: GABA Transporter 1 Antibody [ - Rat hippocampal homogenate showing specific immunolabeling of the~ 67k GAT-1 protein.| Product Name | GABA Transporter 1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to GABA Transporter 1Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl negative channel associated with the GABAA receptor (GABAA-R) subtype. GABA plasma membrane transporters (GATs) influence synaptic neurotransmission by high-affinity uptake and release of GABA. To date, four distinct GABA transporters have been identified: GAT-1, GAT-2, GAT-3, and BGT-1. GAT-1, the most abundant of the transporters, is found predominantly in neurons, but also in some specialized glia (Minelli et al., 1995). GAT-1 is thought to play a key role in epileptogenesis (Zhao et al. 2003). |
| Immunogen | Peptide corresponding to amino acid residues from the C-terminal region of rat GAT-1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| We recommend the following starting dilutions: Western Blot 1:1000 immunohistochemistry 1:100-1:200. Optimal concentration should be determined experimentally by the end user.: |
|
| Species Reactivity | Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | GABA Transporter1 |
|---|---|
| Target | GABA Transporter 1 |
| Molecular Weight(MW) | 67 kDa (note) |
| Tissue Specificity | GAT-1 |
Application
Western Blot: GABA Transporter 1 Antibody [ - Rat hippocampal homogenate showing specific immunolabeling of the~ 67k GAT-1 protein.| Application Notes | We recommend the following starting dilutions: Western Blot 1:1000 immunohistochemistry 1:100-1:200. Optimal concentration should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Storage Buffer | 0.1 ml in 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg per ml BSA and 50% glycerol. |
