Application
GABARAPL1 antibody detects GABARAPL1 protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg whole cell lysate/extract of human GABARAPL1-transfected 293T cells 15 % SDS-PAGE GABARAPL1 antibody dilution: 1:1000Application
GABARAPL1 antibody detects GABARAPL1 protein at autophagosome by immunofluorescent analysis. Samples: HeLa cells mock (left) and treated with 50μM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: GABARAPL1 protein stained by GABARAPL1 antibody diluted at 1:1000. Red: Phalloidin, a F-actin marker.Application
GABARAPL1 antibody detects GABARAPL1 protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg HeLa whole cell lysate/extract 15 % SDS-PAGE GABARAPL1 antibody dilution: 1:500| Product Name | GABARAPL1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GABARAPL1 (GABA(A) receptor-associated protein like 1)Increases cell-surface expression of kappa-type opioid receptor through facilitating anterograde intracellular trafficking of the receptor. |
| Immunogen | Full length human GABARAPL1 Recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, Pig, Rhesus Monkey, Bovine, Guinea pigMouse (100%), Rat (100%), Xenopus laevis (100%), Pig (98%), Rhesus Monkey (100%), Bovine (100%), Guinea pig (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ATG8L ATG8B APG8L GEC1 GABA(A) receptor-associated protein like 1 APG8-LIKE ATG8 |
|---|---|
| Target | GABARAPL1 |
| Molecular Weight(MW) | 14 kDa (note) |
| Cellular Localization | Cytoplasm , Membrane (By similarity) , Endoplasmic reticulum (By similarity) , Golgi apparatus |
Application
GABARAPL1 antibody detects GABARAPL1 protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg whole cell lysate/extract of human GABARAPL1-transfected 293T cells 15 % SDS-PAGE GABARAPL1 antibody dilution: 1:1000
Application
GABARAPL1 antibody detects GABARAPL1 protein at autophagosome by immunofluorescent analysis. Samples: HeLa cells mock (left) and treated with 50μM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: GABARAPL1 protein stained by GABARAPL1 antibody diluted at 1:1000. Red: Phalloidin, a F-actin marker.
Application
GABARAPL1 antibody detects GABARAPL1 protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg HeLa whole cell lysate/extract 15 % SDS-PAGE GABARAPL1 antibody dilution: 1:500| Positive Control | 293T , HeLa , human GABARAPL1-transfected 293T |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
