Application
Various whole cell extracts (60 µg) and tissue extracts (15 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with GABARAPL1 antibody diluted at 1:1000.Application
GABARAPL1 antibody detects GABARAPL1 protein at autophagosome by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GABARAPL1 protein stained by GABARAPL1 antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:50. Blue: Hoechst 33342 staining.| Product Name | GABARAPL1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GABARAPL1 (GABA(A) receptor-associated protein like 1)Increases cell-surface expression of kappa-type opioid receptor through facilitating anterograde intracellular trafficking of the receptor. |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human GABARAPL1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Pig, Rhesus Monkey, Bovine, Guinea pigXenopus laevis (100%), Pig (100%), Rhesus Monkey (100%), Bovine (100%), Guinea pig (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | APG8L antibody APG8LIKE antibody ATG8 antibody ATG8B antibody ATG8L antibody GABA(A) receptor-associated protein like 1 antibody GABARAPL1 antibody GABARAPL1 antibody GEC1 antibody |
|---|---|
| Target | GABARAPL1 |
| Molecular Weight(MW) | 14 kDa (note) |
| Cellular Localization | Cytoplasm , Membrane (By similarity) , Endoplasmic reticulum (By similarity) , Golgi apparatus |
Application
Various whole cell extracts (60 µg) and tissue extracts (15 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with GABARAPL1 antibody diluted at 1:1000.
Application
GABARAPL1 antibody detects GABARAPL1 protein at autophagosome by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GABARAPL1 protein stained by GABARAPL1 antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:50. Blue: Hoechst 33342 staining.| Positive Control | U87MG , SKNSH , mouse brain , rat brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
