Application
Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE diluted at 1:5000Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using GABPB1 antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using GABP-beta1 antibody at 1:100 dilution.| Product Name | GABPB1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GABPB1 (GA binding protein transcription factor, beta subunit 1)This gene encodes the GA-binding protein transcription factor, beta subunit. This protein forms a tetrameric complex with the alpha subunit, and stimulates transcription of target genes. The encoded protein may be involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. The crystal structure of a similar protein in mouse has been resolved as a ternary protein complex. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human GABPB1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Xenopus laevis, Chicken, Rhesus Monkey, BovineMouse (94%), Xenopus laevis (90%), Chicken (94%), Rhesus Monkey (96%), Bovine (96%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | BABPB2 antibody E4TF1 antibody E4TF1-47 antibody E4TF1-53 antibody E4TF1B antibody GABPB antibody GABPB2 antibody NRF2B1 antibody NRF2B2 antibody GABPB1 antibody transcription factor E4TF1-53 antibody GABP subunit beta-2 antibody nuclear respir |
|---|---|
| Molecular Weight(MW) | 42 kDa (note) (4) |
| Cellular Localization | Nucleus |
Application
Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE diluted at 1:5000
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using GABPB1 antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using GABP-beta1 antibody at 1:100 dilution.| Positive Control | 293T , A431 , HeLa , HepG2 , Molt-4 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
