Application
GAD65 antibody detects GAD65 protein expression by immunohistochemical analysis. Sample: Paraffin-Embedded adult mouse retina. Green: GAD65 protein stained by GAD65 antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:250. Blue: Fluoroshield with DAPI .Application
Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD65 antibody diluted at 1:1000.| Product Name | GAD65 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GAD65 (glutamic acid decarboxylase 2)Catalyzes the production of GABA. |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of mouse GAD65. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | MouseRat |
| Predicted Reactivity | Human, Dog, Rhesus Monkey, BovineHuman (83%), Dog (81%), Rhesus Monkey (80%), Bovine (81%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 6330404F12Rik antibody GAD(65) antibody Gad2 antibody glutamic acid decarboxylase 2 antibody |
|---|---|
| Target | GAD65 |
| Molecular Weight(MW) | 65 kDa (note) |
| Cellular Localization | Cytoplasm , cytosol , Cytoplasmic vesicle , Cell junction , synapse , presynaptic cell membrane; Lipid-anchor , Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side , Cytoplasm , cytosol , Cytoplasmic vesicle , Cell junction , synapse |
Application
GAD65 antibody detects GAD65 protein expression by immunohistochemical analysis. Sample: Paraffin-Embedded adult mouse retina. Green: GAD65 protein stained by GAD65 antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:250. Blue: Fluoroshield with DAPI .
Application
Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD65 antibody diluted at 1:1000.| Positive Control | mouse brain , rat brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
