Application
Western blot analysis of extracts of various cell lines, using GAD2 antibody.Application
Immunofluorescence analysis of A549 cell using GAD2 antibody. Blue: DAPI for nuclear staining.| Product Name | GAD65 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GAD65This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2008] |
| Immunogen | Recombinant protein of human GAD2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:10 - 1:100: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | GAD2 glutamate decarboxylase 2 (pancreatic islets and brain 65kDa) |
|---|---|
| Target | GAD65 |
| Molecular Weight(MW) | 65 kDa (note) |
Application
Western blot analysis of extracts of various cell lines, using GAD2 antibody.
Application
Immunofluorescence analysis of A549 cell using GAD2 antibody. Blue: DAPI for nuclear staining.| Application Notes | WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:10 - 1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
