Application
ELISA ImageExpression levels as percentages of hGAD65 in total soluble protein (TSP) of hGAD65 expressing C. reinhardtii transformants. The expression level was determined by hGAD65 specific ELISA as described in detail in methods. hGAD-1 and hGAD-2, two independent algal transformants; WT, untransformed wild type C. reinhardtii 137c strain.| Product Name | GAD67 + GAD65 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GAD67 + GAD65catalyzes synthesis of gamma-Aminobutyric acid (GABA), the most widely distributed inhibitory neurotransmitter [RGD, Feb 2006] |
| Immunogen | Synthetic peptide sequence D-F-L-I-E-E-I-E-R-L-G-Q-D-L from the C terminus of rat GAD65. The peptide sequence is identical to rat GAD67. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHC-FIHC-PWB |
| IHC-Fr: 1:200 - 1:5000, WB: 1:1000 - 1:10000: |
|
| Species Reactivity | HumanMouseRatCat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | Gad2 glutamate decarboxylase 2 gad65 |
|---|---|
| Target | GAD67 + GAD65 |
Application
ELISA ImageExpression levels as percentages of hGAD65 in total soluble protein (TSP) of hGAD65 expressing C. reinhardtii transformants. The expression level was determined by hGAD65 specific ELISA as described in detail in methods. hGAD-1 and hGAD-2, two independent algal transformants; WT, untransformed wild type C. reinhardtii 137c strain.| Application Notes | IHC-Fr: 1:200 - 1:5000, WB: 1:1000 - 1:10000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. For short-term storage, store at 4° C (up to 10 days). For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. This product may contain preciptation. Recommend microcentrifugation before use. |
| Storage Buffer | PBS, 0.09% Sodium Azide |
