Application
Western blot analysis of GADD153 (pS30) expression in HeLa (A); Raw264.7 (B); rat spleen (C) whole cell lysates.| Product Name | GADD153 (phospho Ser30) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GADD153 (phospho Ser30)This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified. [provided by RefSeq, Aug 2010] |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human GADD153. The exact sequence is proprietary. |
| Modification | p-Ser30 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: 1:500 - 1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DNA-damage-inducible transcript 3 CHOP10 CHOP CHOP-10 DDIT3 GADD153 CEBPZ |
|---|---|
| Target | GADD153 |
| Tissue Specificity | Recognizes endogenous levels of GADD153 (pS30) protein. |
Application
Western blot analysis of GADD153 (pS30) expression in HeLa (A); Raw264.7 (B); rat spleen (C) whole cell lysates.| Application Notes | WB: 1:500 - 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4 C. Upon delivery aliquot and store at -20 C for one year. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
