Application
Western blot analysis of extracts from Jurkat cells treated with PMA using Phospho-CHOP (Ser30) antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | GADD153 (phospho Ser30) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GADD153 (phospho Ser30)This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified. [provided by RefSeq, Aug 2010] |
| Immunogen | Peptide sequence around phosphorylation site of serine30(V-L-S(p)-S-D) derived from Human CHOP . |
| Modification | p-Ser30 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: 1:500 - 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CHOP-10 GADD153 DNA-damage-inducible transcript 3 CEBPZ CHOP10 CHOP DDIT3 |
|---|---|
| Target | GADD153 (phospho Ser30) |
| Tissue Specificity | The antibody detects endogenous level of CHOP only when phosphorylated at serine 30. |
Application
Western blot analysis of extracts from Jurkat cells treated with PMA using Phospho-CHOP (Ser30) antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | WB: 1:500 - 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20˚C/1 year |
| Storage Buffer | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
