| Product Name | GADD153 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to DDIT3DDIT3 is a basic region leucine zipper transcription factor, devoid of DNA binding site. It is capable of heterodimerizing with members of the CCAAT/ enhancer binding protein (C/EBP) family of transcription factors. It is both an activating and a repressing factor that is markedly induced in response to many signals such as growth arrest, DNA damage, calcium ionophore, nutrient deprivation, hypoxia, hyperoxia, hyperosmosis, and endoplasmic reticulum stress. |
| Immunogen | Synthetic peptide: KEATRRALIDRMVNLHQA conjugated to KLH, corresponding to amino acids 153-169 of Human DDIT3 (with added N-terminal K). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB: a working dilution of 1:200-1:400 is determined using a whole extract of HEK-293 over-expressing GADD153 and a chemiluminescent detection system.ICC/IF: a working dilution of 5-10 ug/ml is determined using Hela human cells. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DDIT3 126337 MGC4154 CEBPZ P35638 CHOP CHOP10 GADD153 1649 |
|---|---|
| Target | DDIT3 |
| Cellular Localization | Nuclear |
| Positive Control | overexpressed GADD153 in 293T , HeLa |
|---|---|
| Application Notes | WB: a working dilution of 1:200-1:400 is determined using a whole extract of HEK-293 over-expressing GADD153 and a chemiluminescent detection system.ICC/IF: a working dilution of 5-10 ug/ml is determined using Hela human cells. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at −20°C. |
| Storage Buffer | Preservative: 15mM Sodium Azide; Constituents: 1% BSA, 0.01M PBS. pH 7.4 |
