Application
Immunofluorescence analysis of A549 cell using DDIT3 antibody. Blue: DAPI for nuclear staining.Application
Immunohistochemistry of paraffin-embedded mouse lung using DDIT3 antibody at dilution of 1:100 (x400 lens).| Product Name | GADD153 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GADD153This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified. [provided by RefSeq, Aug 2010] |
| Immunogen | Recombinant protein of human DDIT3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIHC-PWB |
| WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:50 - 1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CHOP-10 DDIT3 CEBPZ DNA-damage-inducible transcript 3 CHOP CHOP10 |
|---|---|
| Target | GADD153 |
| Molecular Weight(MW) | 19 kDa (note) |
Application
Immunofluorescence analysis of A549 cell using DDIT3 antibody. Blue: DAPI for nuclear staining.
Application
Immunohistochemistry of paraffin-embedded mouse lung using DDIT3 antibody at dilution of 1:100 (x400 lens).| Application Notes | WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:50 - 1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
