Application
Sample (30 ug of whole cell lysate) A: HeLa B: HeLa treated tunicaycin 8 hr 12% SDS PAGE diluted at 1:500Application
GADD153 antibody detects GADD153 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GADD153 protein stained by GADD153 antibody diluted at 1:200. Red: phalloidin, a cytoskeleton marker, diluted at 1:50. Scale bar = 10 μm.| Product Name | GADD153 antibody |
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| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GADD153 (DNA-damage-inducible transcript 3)This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified. [provided by RefSeq] |
| Immunogen | Full length human GADD153 Recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Pig, Rhesus Monkey, BovineMouse (88%), Rat (90%), Pig (95%), Rhesus Monkey (98%), Bovine (92%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CEBPZ antibody CHOP antibody CHOP-10 antibody CHOP10 antibody GADD153 antibody MGC4154 antibody DDIT3 antibody DNA damage-inducible transcript 3 protein antibody growth arrest and DNA damage-inducible protein GADD153 antibody c/EBP-homologous prot |
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| Molecular Weight(MW) | 19 kDa (note) (6) |
| Cellular Localization | Nucleus |
Application
Sample (30 ug of whole cell lysate) A: HeLa B: HeLa treated tunicaycin 8 hr 12% SDS PAGE diluted at 1:500
Application
GADD153 antibody detects GADD153 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GADD153 protein stained by GADD153 antibody diluted at 1:200. Red: phalloidin, a cytoskeleton marker, diluted at 1:50. Scale bar = 10 μm.| Positive Control | HeLa , HeLa treated tunicaycin 8 hr |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
