Application
WB analysis of HepG2 cells using GADD45B antibody at 0.2-1μg/ml.Application
IHC-P analysis of human prostate cancer tissue using GADD45B antibody at 2μg/ml. Leica Bond antigen retrieval solution 1 (citrate buffer, pH 6.0) for 20 min at 99oCApplication
IHC-P analysis of human prostate cancer tissue using GADD45B antibody at 2μg/ml. Leica Bond antigen retrieval solution 1 (citrate buffer, pH 6.0) for 20 min at 99oC.Application
WB analysis of human fetal lung tissue using GADD45B antibody at 3μg/ml.| Product Name | GADD45B antibody, Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GADD45BThis gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The genes in this group respond to environmental stresses by mediating activation of the p38/JNK pathway. This activation is mediated via their proteins binding and activating MTK1/MEKK4 kinase, which is an upstream activator of both p38 and JNK MAPKs. The function of these genes or their protein products is involved in the regulation of growth and apoptosis. These genes are regulated by different mechanisms, but they are often coordinately expressed and can function cooperatively in inhibiting cell growth. [provided by RefSeq, Jul 2008] |
| Immunogen | A synthetic peptide corresponding to an Internal region of Human GADD45B |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Predicted Reactivity | Cow: 100%; Dog: 100%; Guinea Pig: 92%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rat: 100% |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | GADD45B MYD118 GADD45BETA |
|---|---|
| Target | GADD45B |
| Molecular Weight(MW) | 18 KDa (note) |
Application
WB analysis of HepG2 cells using GADD45B antibody at 0.2-1μg/ml.
Application
IHC-P analysis of human prostate cancer tissue using GADD45B antibody at 2μg/ml. Leica Bond antigen retrieval solution 1 (citrate buffer, pH 6.0) for 20 min at 99oC
Application
IHC-P analysis of human prostate cancer tissue using GADD45B antibody at 2μg/ml. Leica Bond antigen retrieval solution 1 (citrate buffer, pH 6.0) for 20 min at 99oC.
Application
WB analysis of human fetal lung tissue using GADD45B antibody at 3μg/ml.| Positive Control | HepG2 , human fetal lung |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. For short-term storage, store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
