Application
Sample (30 µg of whole cell lysate) A: NCI-H929 15% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Whole cell extract (30 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with Galectin 1 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Galectin 1 antibody at 1:500 dilution.Application
Galectin 1 antibody detects Galectin 1 protein at cytoplasm by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Galectin 1 protein stained by Galectin 1 antibody diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
Galectin 1 antibody detects Galectin 1 protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: Galectin 1 protein stained by Galectin 1 antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500. Blue: Fluoroshield with DAPI .Application
Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Galectin 1 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (30 µg of whole cell lysate) A: BCL-1 15% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | Galectin 1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Galectin 1 (lectin, galactoside-binding, soluble, 1)The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. This gene product may act as an autocrine negative growth factor that regulates cell proliferation. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Galectin 1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-FIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Dog, Pig, Sheep, Rhesus Monkey, BovineDog (81%), Pig (84%), Sheep (84%), Rhesus Monkey (97%), Bovine (85%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp686E23103 antibody GAL1 antibody GBP antibody LGALS1 antibody HPL antibody gal-1 antibody lactose-binding lectin 1 antibody galaptin antibody 14 kDa laminin-binding protein antibody HLBP14 antibody S-Lac lectin 1 antibody beta-galactoside-b |
|---|---|
| Molecular Weight(MW) | 15 kDa (note) |
| Cellular Localization | Secreted , extracellular space , extracellular matrix |
Application
Sample (30 µg of whole cell lysate) A: NCI-H929 15% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Whole cell extract (30 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with Galectin 1 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Galectin 1 antibody at 1:500 dilution.
Application
Galectin 1 antibody detects Galectin 1 protein at cytoplasm by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Galectin 1 protein stained by Galectin 1 antibody diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
Galectin 1 antibody detects Galectin 1 protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: Galectin 1 protein stained by Galectin 1 antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500. Blue: Fluoroshield with DAPI .
Application
Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Galectin 1 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (30 µg of whole cell lysate) A: BCL-1 15% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | HeLa , NCI-H929 , BCL-1 , PC-12 , Rat2 , *U2OS |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
