Application
GALNS antibody detects GALNS protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract B. 30 µg Rat2 whole cell lysate/extract 7.5% SDS-PAGE GALNS antibody dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
GALNS antibody detects GALNS protein by western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg C2C12 whole cell lysate/extract 7.5% SDS-PAGE GALNS antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using GALNS antibody at 1:100 dilution.Application
Sample (30 µg of whole cell lysate) A: H1299 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using GALNS antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:500.| Product Name | GALNS antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GALNS (galactosamine (N-acetyl)-6-sulfate sulfatase)This gene encodes N-acetylgalactosamine-6-sulfatase which is a lysosomal exohydrolase required for the degradation of the glycosaminoglycans, keratan sulfate, and chondroitin 6-sulfate. Sequence alterations including point, missense and nonsense mutations, as well as those that affect splicing, result in a deficiency of this enzyme. Deficiencies of this enzyme lead to Morquio A syndrome, a lysosomal storage disorder. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human GALNS. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Zebrafish, Xenopus laevis, Xenopus Tropicalis, Dog, Pig, BovineZebrafish (81%), Xenopus laevis (84%), Xenopus Tropicalis (80%), Dog (92%), Pig (91%), Bovine (91%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ17434 antibody FLJ42844 antibody FLJ98217 antibody GALNAC6S antibody GAS antibody MPS4A antibody GALNS antibody galactose-6-sulfate sulfatase antibody chondroitinase antibody N-acetylgalactosamine-6-sulfate sulfatase antibody galNAc6S sulfatas |
|---|---|
| Molecular Weight(MW) | 58 kDa (note) (2) |
| Cellular Localization | Lysosome |
Application
GALNS antibody detects GALNS protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract B. 30 µg Rat2 whole cell lysate/extract 7.5% SDS-PAGE GALNS antibody dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
GALNS antibody detects GALNS protein by western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg C2C12 whole cell lysate/extract 7.5% SDS-PAGE GALNS antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using GALNS antibody at 1:100 dilution.
Application
Sample (30 µg of whole cell lysate) A: H1299 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using GALNS antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:500.| Positive Control | H1299 , Neuro2A , C2C12 , PC-12 , Rat2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
