Application
GALNT2 antibody detects GALNT2 protein by western blot analysis. A. 50 µg rat brain lysate/extract 7.5% SDS-PAGE GALNT2 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample(30 µg whole cell lysate) A:MOLT4 7.5% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (50 µg of whole cell lysate) A: Mouse white adipose tissue 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using GALNT2 antibody at 1:250 dilution.Application
GALNT2 antibody [N1C1] detects GALNT2 protein at Golgi apparatus by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: GALNT2 protein stained by GALNT2 antibody [N1C1] diluted at 1:500. Blue: Hoechst 33343 staining.| Product Name | GALNT2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GALNT2 (UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 2 (GalNAc-T2))This gene encodes polypeptide N-acetylgalactosaminyltransferase 2, a member of the GalNAc-transferases family. This family transfers an N-acetyl galactosamine to the hydroxyl group of a serine or threonine residue in the first step of O-linked oligosaccharide biosynthesis. Individual GalNAc-transferases have distinct activities and initiation of O-glycosylation in a cell is regulated by a repertoire of GalNAc-transferases. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human GALNT2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Zebrafish, Rhesus Monkey, BovineZebrafish (92%), Rhesus Monkey (100%), Bovine (99%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | GalNAc-T2 antibody, GALNT2 antibody, polypeptide GalNAc transferase 2 antibody, UDP-GalNAc transferase 2 antibody, polypeptide N-acetylgalactosaminyltransferase 2 antibody, pp-GaNTase 2 antibody, UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 2 a |
|---|---|
| Molecular Weight(MW) | 65 kDa (note) (2) |
| Cellular Localization | Golgi apparatus , Golgi stack membrane; Single-pass type II membrane protein , Secreted |
Application
GALNT2 antibody detects GALNT2 protein by western blot analysis. A. 50 µg rat brain lysate/extract 7.5% SDS-PAGE GALNT2 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample(30 µg whole cell lysate) A:MOLT4 7.5% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (50 µg of whole cell lysate) A: Mouse white adipose tissue 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using GALNT2 antibody at 1:250 dilution.
Application
GALNT2 antibody [N1C1] detects GALNT2 protein at Golgi apparatus by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: GALNT2 protein stained by GALNT2 antibody [N1C1] diluted at 1:500. Blue: Hoechst 33343 staining.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
