Application
Western blot analysis of GALNT3 Antibody (Center) in MCF-7 cell line lysates (35ug/lane). GALNT3 (arrow) was detected using the purified Pab.Application
GALNT3 Antibody (Center) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human skin tissue reacted with GALNT3 Antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry.| Product Name | GALNT3 antibody, Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to GALNT3 (Internal)This gene encodes UDP-GalNAc transferase 3, a member of the GalNAc-transferases family. This family transfers an N-acetyl galactosamine to the hydroxyl group of a serine or threonine residue in the first step of O-linked oligosaccharide biosynthesis. Individual GalNAc-transferases have distinct activities and initiation of O-glycosylation is regulated by a repertoire of GalNAc-transferases. The protein encoded by this gene is highly homologous to other family members, however the enzymes have different substrate specificities. [provided by RefSeq] |
| Immunogen | KLH conjugated synthetic peptide between 461~490 amino acids from the Center region of human GALNT3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| ELISA: Use at 1:1,000 | |
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ProteinUDP acetylgalactosaminyltransferase 3, Polypeptide N-acetylgalactosaminyltransferase 3, Polypeptide Nacetylgalactosaminyltransferase 3, Polypeptide Nacetylgalactosaminyltransferase3, ProteinUDP acetylgalactosaminyltransferase3, GalNAc-T3, Polypeptid |
|---|---|
| Target | GALNT3 |
| Molecular Weight(MW) | 72.61 kDa (note) |
| Cellular Localization | Resides preferentially in the trans and medial parts of the Golgi stack. |
Application
Western blot analysis of GALNT3 Antibody (Center) in MCF-7 cell line lysates (35ug/lane). GALNT3 (arrow) was detected using the purified Pab.
Application
GALNT3 Antibody (Center) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human skin tissue reacted with GALNT3 Antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry.| Application Notes | ELISA: Use at 1:1,000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
