Application
Sample(30 ug whole cell lysate) A:A431 7.5% SDS PAGE diluted at 1:1000Application
Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GALNT6 antibody [N1C2] diluted at 1:1000.Application
Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using GALNT6 antibody at 1:500 dilution.Application
Immunofluorescence analysis of methanol-fixed A431, using GALNT6 antibody at 1:200 dilution.| Product Name | GALNT6 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GALNT6 (UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 6 (GalNAc-T6))This gene encodes a member of the UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase (GalNAc-T) family of enzymes. GalNAc-Ts initiate mucin-type O-linked glycosylation in the Golgi apparatus by catalyzing the transfer of GalNAc to serine and threonine residues on target proteins. They are characterized by an N-terminal transmembrane domain, a stem region, a lumenal catalytic domain containing a GT1 motif and Gal/GalNAc transferase motif, and a C-terminal ricin/lectin-like domain. GalNAc-Ts have different, but overlapping, substrate specificities and patterns of expression. The encoded protein is capable of glycosylating fibronectin peptide in vitro and is expressed in a fibroblast cell line, indicating that it may be involved in the synthesis of oncofetal fibronectin. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human GALNT6. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanRat |
| Predicted Reactivity | Mouse, Chicken, BovineMouse (92%), Chicken (80%), Bovine (92%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | GALNAC-T6 antibody, GalNAcT6 antibody, GALNT6 antibody, GalNAc transferase 6 antibody, polypeptide N-acetylgalactosaminyltransferase 6 antibody, protein-UDP acetylgalactosaminyltransferase 6 antibody, pp-GaNTase 6 antibody, UDP-GalNAc:polypeptide N-acetylg |
|---|---|
| Molecular Weight(MW) | 71 kDa (note) |
| Cellular Localization | Golgi apparatus membrane; Single-pass type II membrane protein |
Application
Sample(30 ug whole cell lysate) A:A431 7.5% SDS PAGE diluted at 1:1000
Application
Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GALNT6 antibody [N1C2] diluted at 1:1000.
Application
Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using GALNT6 antibody at 1:500 dilution.
Application
Immunofluorescence analysis of methanol-fixed A431, using GALNT6 antibody at 1:200 dilution.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
