Application
Western blot analysis of extracts from HT-29, MCF-7 and C2C12 cells using g-Catenin Antibody| Product Name | gamma Catenin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to gamma CateninCommon junctional plaque protein. The membrane-associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE-cadherin function in endothelial cells. Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton |
| Immunogen | Peptide sequence around aa.726~730(I-D-T-Y-S) derived from Human gamma -Catenin |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. | |
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ARVD12, CTNNG, DP3, DPIII, PDGB, PKGB, JUP, catenin gamma, catenin (cadherin-associated protein), gamma 80kDa, desmoplakin-3, catenin (cadherin-associated protein), gamma (80kD), gamma-catenin, junction plakoglobin, desmoplakin III |
|---|---|
| Target | gamma Catenin |
| Molecular Weight(MW) | 83 (note) |
Application
Western blot analysis of extracts from HT-29, MCF-7 and C2C12 cells using g-Catenin Antibody| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
