Application
Immunofluorescence staining of mouse fibroblasts using anti-gamma-tubulin (TU-30; direct conjugate with Dyomics 547, red). Nuclei were stained with DAPI (blue).Application
Immunofluorescence staining of microtubular networks in 3T3 mouse fibroblasts. 3A – metaphase; 3B – anaphase; 3C - telophase Gamma-tubulin (red) stained with anti-gamma-tubulin (TU-30) , alpha-tubulin (green) with polyclonal anti-alpha-tubulin antibody and nuclei with DAPI (blue).Application
Immunofluorescence staining of P19X1 mouse embryonal carcinoma cell line using anti-gamma-tubulin (TU-30) (detection by secondary antibody Goat anti-mouse Cy3). Nuclei were stained with DAPI (blue).| Product Name | gamma tubulin antibody [TU-30] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [TU-30] to gamma tubulinThe gamma-tubulin (TUBG1; relative molecular weight about 48 kDa) is a minor member of tubulin family (less that 0.01% of tubulin dimer). The gamma-tubulin ring structures, however, serve to provide structural primer for initiation of microtubular nucleation and growth, thereby being crutial for microtubule-based cellular processes, above all for mitotic spindle formation. In animal cells, a center of microtubule organization is the centrosome composed of a pair of cylindrical centrioles surrounded by fibrous pericentriolar material containing gamma-tubulin. Formation of the mitotic spindle is preceded by duplication of centrosome during S phase. Before mitosis, both centrosomes increase their microtubule nucleation capacity and form two microtuble asters that are pushed apart from each other by the forces of motor proteins associated at the microtubule surface.Entrez Gene (human): 17q21The Human Protein Reference Database |
| Immunogen | C-terminal peptide of gamma-tubulin counjugated to KLH. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | TU-30 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| ICC/IF: Use at Purified Antibody: 1-2 µg/mL. Staining technique: (a) Fix cells for 10 min in methanol at -20 degree and for 6 min in acetone at -20 degree | |
| Species Reactivity | PlantProtozoaInvertebratesVertebrates |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | TUBG |
|---|---|
| Target | gamma-tubulin |
| Tissue Specificity | The antibody TU-30 recognizes C-terminal peptide sequence EYHAATRPDYISWGTQ (aa 434-449) of gamma-tubulin, a 48 kDa structural constituent of cytoskeleton and microtubule organizing center (MTOC). |
Application
Immunofluorescence staining of mouse fibroblasts using anti-gamma-tubulin (TU-30; direct conjugate with Dyomics 547, red). Nuclei were stained with DAPI (blue).
Application
Immunofluorescence staining of microtubular networks in 3T3 mouse fibroblasts. 3A – metaphase; 3B – anaphase; 3C - telophase Gamma-tubulin (red) stained with anti-gamma-tubulin (TU-30) , alpha-tubulin (green) with polyclonal anti-alpha-tubulin antibody and nuclei with DAPI (blue).
Application
Immunofluorescence staining of P19X1 mouse embryonal carcinoma cell line using anti-gamma-tubulin (TU-30) (detection by secondary antibody Goat anti-mouse Cy3). Nuclei were stained with DAPI (blue).| Application Notes | ICC/IF: Use at Purified Antibody: 1-2 µg/mL. Staining technique: (a) Fix cells for 10 min in methanol at -20 degree and for 6 min in acetone at -20 degree |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 2-8°C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20°C. Avoid freeze/thaw cycles. |
| Storage Buffer | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
