Application
GAP43 antibody detects Gap43 protein by western blot analysis. A. 1 µg Rat brain lysate/extract 12% SDS-PAGE GAP43 antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
GAP43 antibody detects Gap43 protein by western blot analysis. A. 5 µg mouse brain lysate/extract 12% SDS-PAGE GAP43 antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
GAP43 antibody detects Gap43 protein by western blot analysis. A. 30 µg SK-N-SH whole cell lysate/extract B. 30 µg IMR32 whole cell lysate/extract C. 30 µg SK-N-AS whole cell lysate/extract 12% SDS-PAGE GAP43 antibody dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
GAP43 antibody detects Gap43 protein at on Cal27 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Cal27 xenograft. GAP43 antibody dilution: 1:500.Application
GAP43 antibody detects GAP43 protein at membrane by immunofluorescent analysis. Sample: SKNSH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GAP43 protein stained by GAP43 antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | GAP43 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GAP43 (growth associated protein 43)may play a role in neurite outgrowth and nerve regeneration [RGD] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of rat GAP43. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Pig, ChimpanzeePig (80%), Chimpanzee (90%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | axonal membrane protein GAP-43 antibody, neuromodulin antibody, brain abundant, membrane attached signal protein 2 antibody, growth-associated protein 43 antibody, growth accentuating protein 43 antibody, protein F1 antibody, Basp2 antibody, MGC156666 anti |
|---|---|
| Molecular Weight(MW) | 24 kDa (note) |
| Cellular Localization | Cell membrane; Peripheral membrane protein; Cytoplasmic side , Cell projection , growth cone membrane; Peripheral membrane protein; Cytoplasmic side , Cell junction , synapse |
Application
GAP43 antibody detects Gap43 protein by western blot analysis. A. 1 µg Rat brain lysate/extract 12% SDS-PAGE GAP43 antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
GAP43 antibody detects Gap43 protein by western blot analysis. A. 5 µg mouse brain lysate/extract 12% SDS-PAGE GAP43 antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
GAP43 antibody detects Gap43 protein by western blot analysis. A. 30 µg SK-N-SH whole cell lysate/extract B. 30 µg IMR32 whole cell lysate/extract C. 30 µg SK-N-AS whole cell lysate/extract 12% SDS-PAGE GAP43 antibody dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
GAP43 antibody detects Gap43 protein at on Cal27 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Cal27 xenograft. GAP43 antibody dilution: 1:500.
Application
GAP43 antibody detects GAP43 protein at membrane by immunofluorescent analysis. Sample: SKNSH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GAP43 protein stained by GAP43 antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
