Application
Immunofluorescence staining of methanol-fixed Hela cells using GAP43 Antibody .Application
Western blot analysis of extracts from mouse brain tissue using GAP43(Ab-41) Antibody and the same antibody preincubated with blocking peptide.Application
Western blot analysis of extracts from mouse brain tissue using GAP43 Antibody and the same antibody preincubated with blocking peptide.Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GAP43 Antibody (left) or the same antibody preincubated with blocking peptide (right).| Product Name | GAP43 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GAP43GAP43 encoded by this gene has been termed a 'growth' or 'plasticity' protein because it is expressed at high levels in neuronal growth cones during development and axonal regeneration. This protein is considered a crucial component of an effective regenerative response in the nervous system. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. |
| Immunogen | Peptide sequence around aa.38~43 (Q-A-S-F-R) derived from Human GAP43. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | B-50, PP46, GAP43, calmodulin-binding protein P-57, nerve growth-related peptide GAP43, neural phosphoprotein B-50, axonal membrane protein GAP-43, neuromodulin, growth-associated protein 43, neuron growth-associated protein 43, protein F1, growth associat |
|---|---|
| Target | GAP43 |
| Molecular Weight(MW) | 43 (note) |
Application
Immunofluorescence staining of methanol-fixed Hela cells using GAP43 Antibody .
Application
Western blot analysis of extracts from mouse brain tissue using GAP43(Ab-41) Antibody and the same antibody preincubated with blocking peptide.
Application
Western blot analysis of extracts from mouse brain tissue using GAP43 Antibody and the same antibody preincubated with blocking peptide.
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GAP43 Antibody (left) or the same antibody preincubated with blocking peptide (right).| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
