Application
Sample (30 ug of whole cell lysate) A: IMR32 12% SDS PAGE diluted at 1:1000Application
Rat tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAP43 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAP43 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | GAP43 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GAP43 (growth associated protein 43)The protein encoded by this gene has been termed a 'growth' or 'plasticity' protein because it is expressed at high levels in neuronal growth cones during development and axonal regeneration. This protein is considered a crucial component of an effective regenerative response in the nervous system. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human GAP43. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Cat, Pig, Chimpanzee, BovineCat (85%), Pig (80%), Chimpanzee (100%), Bovine (88%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | B-50 antibody, PP46 antibody, GAP43 antibody, calmodulin-binding protein P-57 antibody, nerve growth-related peptide GAP43 antibody, neural phosphoprotein B-50 antibody, axonal membrane protein GAP-43 antibody, neuromodulin antibody, growth-associated prot |
|---|---|
| Molecular Weight(MW) | 25 kDa (note) (2) |
| Cellular Localization | Cell membrane; Peripheral membrane protein; Cytoplasmic side , Cell projection , growth cone membrane; Peripheral membrane protein; Cytoplasmic side , Cell junction , synapse |
Application
Sample (30 ug of whole cell lysate) A: IMR32 12% SDS PAGE diluted at 1:1000
Application
Rat tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAP43 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAP43 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
