Application
Whole zebrafish extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Gapdh antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using gapdh antibody at 1:300 dilution.