Application
Western blot of rat heart protein extract usingApplication
WB analysis of HeLa, 293, HUVEC and PC12 lysates (25 ug per lane) using GAPDH antibody at a dilution of 1:3,000.| Product Name | GAPDH antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GAPDHGlyceraldehyde 3 phosphate dehydrogenase (GAPDH) is an enzyme involved in glycolysis. In addition to this role, it is involved in other cellular processes ranging from membrane fusion, neuronal apoptosis to cancer. GAPDH is expressed at high levels in most tissues and is therefore useful as protein loading control in western blot analysis. Additionally, the antigen is conserved in most eukaryotes. |
| Immunogen | Synthetic peptide: V(126) M G V N H E K Y(140) |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: 1:1000 - 1:5000 Optimal dilution / concentration should be determined by the end user. | |
| Species Reactivity | HumanMouseRatZebra Fish |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | P04406, G3PD, 138400, MGC88685, 2597, GAPD, GAPDH |
|---|---|
| Target | GAPDH |
Application
Western blot of rat heart protein extract using
Application
WB analysis of HeLa, 293, HUVEC and PC12 lysates (25 ug per lane) using GAPDH antibody at a dilution of 1:3,000.| Application Notes | WB: 1:1000 - 1:5000 Optimal dilution / concentration should be determined by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 1 mg/ml BSA and 0.05% sodium azide. |
