Application
Sample (30 ug of whole cell lysate) A: MCF-7 10% SDS PAGE diluted at 1:1000Application
Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAPDHS antibody diluted at 1:500.Application
Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE diluted at 1:5000Application
GAPDHS antibody detects GAPDHS protein at cytosol on human by immunohistochemical analysis. Sample: Paraffin-embedded gastric cancer. GAPDHS antibody dilution: 1:500.Application
Immunofluorescence analysis of methanol-fixed MCF-7, using GAPDHS antibody at 1:200 dilution.| Product Name | GAPDHS antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GAPDHS (glyceraldehyde-3-phosphate dehydrogenase, spermatogenic)This gene encodes a protein belonging to the glyceraldehyde-3-phosphate dehydrogenase family of enzymes that play an important role in carbohydrate metabolism. Like its somatic cell counterpart, this sperm-specific enzyme functions in a nicotinamide adenine dinucleotide-dependent manner to remove hydrogen and add phosphate to glyceraldehyde 3-phosphate to form 1,3-diphosphoglycerate. During spermiogenesis, this enzyme may play an important role in regulating the switch between different energy-producing pathways, and it is required for sperm motility and male fertility. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human GAPDHS. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Zebrafish, BovineRat (90%), Zebrafish (82%), Bovine (93%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | GAPD2 antibody, GAPDH-2 antibody, GAPDS antibody, HSD-35 antibody, GAPDHS antibody, spermatogenic glyceraldehyde-3-phosphate dehydrogenase antibody, glyceraldehyde-3-phosphate dehydrogenase, testis-specific antibody, spermatogenic cell-specific glyceraldeh |
|---|---|
| Molecular Weight(MW) | 45 kDa (note) (2) |
| Cellular Localization | Cytoplasm |
Application
Sample (30 ug of whole cell lysate) A: MCF-7 10% SDS PAGE diluted at 1:1000
Application
Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAPDHS antibody diluted at 1:500.
Application
Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE diluted at 1:5000
Application
GAPDHS antibody detects GAPDHS protein at cytosol on human by immunohistochemical analysis. Sample: Paraffin-embedded gastric cancer. GAPDHS antibody dilution: 1:500.
Application
Immunofluorescence analysis of methanol-fixed MCF-7, using GAPDHS antibody at 1:200 dilution.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
