Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GAS7 monoclonal antibody.Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GAS7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GAS7.Application
Anti-GAS7 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with GAS7Application
HEK293T cells transfected with either RC215256 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-GAS7 antibody, and then analyzed by flow cytometry.| Product Name | GAS7 antibody [2D11] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [2D11] to GAS7May play a role in promoting maturation and morphological differentiation of cerebellar neurons. |
| Immunogen | Full length human recombinant protein of human GAS7 (NP_003635) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 2D11 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFWB |
| Flow Cytometry 1:100 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | GAS7, KIAA0394, MGC1348 |
|---|---|
| Target | GAS7 |
| Tissue Specificity | is specific for human GAS7 (NP_003635). |
Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GAS7 monoclonal antibody.
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GAS7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GAS7.
Application
Anti-GAS7 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with GAS7
Application
HEK293T cells transfected with either RC215256 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-GAS7 antibody, and then analyzed by flow cytometry.| Application Notes | Flow Cytometry 1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
