Application
Western blot analysis of extracts from HL60 cells untreated or treated with PMA using GATA1 (Phospho-Ser142) AntibodyApplication
Western blot analysis of extracts from HL60 cells untreated or treated with PMA using GATA1 (Phospho-Ser142) Antibody .Application
Immunofluorescence staining of methanol-fixed Hela cells using GATA1 (Phospho-Ser142) Antibody .Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GATA1 (Phospho-Ser142) Antibody (left) or the same antibody preincubated with blocking peptide (right).| Product Name | GATA1 (phospho Ser142) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GATA1 (Phospho Ser142)GATA1 encodes a protein which belongs to the GATA family of transcription factors. The protein plays an important role in erythroid development by regulating the switch of fetal hemoglobin to adult hemoglobin. Mutations in this gene have been associated with X-linked dyserythropoietic anemia and thrombocytopenia. |
| Immunogen | Peptide sequence around phosphorylation site of serine 142 (R-L-S(p)-P-D) derived from Human GATA1. |
| Modification | p-Ser142 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ERYF1, GATA-1, GF-1, GF1, NFE1, XLTT, GATA1, GATA-binding factor 1, transcription factor GATA1, erythroid transcription factor, globin transcription factor 1, NF-E1 DNA-binding protein, erythroid transcription factor 1, GATA binding protein 1 (globin trans |
|---|---|
| Target | GATA1 (Phospho Ser142) |
| Molecular Weight(MW) | 43 (note) |
Application
Western blot analysis of extracts from HL60 cells untreated or treated with PMA using GATA1 (Phospho-Ser142) Antibody
Application
Western blot analysis of extracts from HL60 cells untreated or treated with PMA using GATA1 (Phospho-Ser142) Antibody .
Application
Immunofluorescence staining of methanol-fixed Hela cells using GATA1 (Phospho-Ser142) Antibody .
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GATA1 (Phospho-Ser142) Antibody (left) or the same antibody preincubated with blocking peptide (right).| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
