Application
WB analysis of lysates from HEK293T cells overexpressing GATA3-DDK (right) or empty vector control (left) using GATA3 antibody at a dilution of 1:1000.Application
ICC/IF analysis of MCF7 (left) and SH-SY5Y cells (right) using GATA3 antibody at a dilution of 1:50 (green). F-Actin (red) was stained with Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.Application
WB analysis of SH-SY5Y whole cell lysate (WCL) and SH-SY5Y nuclear extract (NE) using GATA3 antibody at a dilution of 1:1000.Application
IHC-P analysis of human tonsil tissue with (right) or without (left) GATA3 antibody at a dilution of 1:200. To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min.| Product Name | GATA3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to GATA3This gene encodes a protein which belongs to the GATA family of transcription factors. The protein contains two GATA-type zinc fingers and is an important regulator of T-cell development and plays an important role in endothelial cell biology. Defects in this gene are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia. [provided by RefSeq, Nov 2009] |
| Immunogen | Purified partial recombinant GATA3 protein expressed in E. coli |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Synonyms | GATA binding protein 3 , HDR , HDRS |
|---|---|
| Target | GATA3 |
Application
WB analysis of lysates from HEK293T cells overexpressing GATA3-DDK (right) or empty vector control (left) using GATA3 antibody at a dilution of 1:1000.
Application
ICC/IF analysis of MCF7 (left) and SH-SY5Y cells (right) using GATA3 antibody at a dilution of 1:50 (green). F-Actin (red) was stained with Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.
Application
WB analysis of SH-SY5Y whole cell lysate (WCL) and SH-SY5Y nuclear extract (NE) using GATA3 antibody at a dilution of 1:1000.
Application
IHC-P analysis of human tonsil tissue with (right) or without (left) GATA3 antibody at a dilution of 1:200. To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min.| Form | Liquid |
|---|---|
| Storage Instructions | -20° C |
| Storage Buffer | PBS with 1mg/ml BSA, 30% glycerol |
