Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using GBA antibody at 1:500 dilution.Application
GBA antibody detects GBA protein by Western blot analysis. A. 30 µg HepG2 whole cell lysate/extract 10 % SDS-PAGE GBA antibody dilution: 1:1000| Product Name | GBA antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GBA (glucosidase, beta, acid)This gene encodes a lysosomal membrane protein that cleaves the beta-glucosidic linkage of glycosylceramide, an intermediate in glycolipid metabolism. Mutations in this gene cause Gaucher disease, a lysosomal storage disease characterized by an accumulation of glucocerebrosides. A related pseudogene is approximately 12 kb downstream of this gene on chromosome 1. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human GBA. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Pig, Chimpanzee, BovineMouse (84%), Rat (85%), Pig (90%), Chimpanzee (100%), Bovine (89%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | GBA1 antibody, GCB antibody, GLUC antibody, GBA antibody, imiglucerase antibody, lysosomal glucocerebrosidase antibody, glucosylceramidase antibody, acid beta-glucosidase antibody, beta-glucocerebrosidase antibody, D-glucosyl-N-acylsphingosine glucohydrola |
|---|---|
| Molecular Weight(MW) | 60 kDa (note) (5) |
| Cellular Localization | Lysosome membrane; Peripheral membrane protein; Lumenal side |
Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using GBA antibody at 1:500 dilution.
Application
GBA antibody detects GBA protein by Western blot analysis. A. 30 µg HepG2 whole cell lysate/extract 10 % SDS-PAGE GBA antibody dilution: 1:1000| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
