Application
Sample (30 ug of whole cell lysate) A: Hela 5% SDS PAGE GBF1 antibody diluted at 1:1000Application
GBF1 antibody [N1], N-term detects GBF1 protein at cytoplasm on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. GBF1 antibody [N1], N-term dilution: 1:500.Application
Immunofluorescence analysis of methanol-fixed HeLa, using GBF1 antibody at 1:200 dilution.| Product Name | GBF1 antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GBF1 (golgi brefeldin A resistant guanine nucleotide exchange factor 1)This gene encodes a member of the Sec7 domain family. The encoded protein is a guanine nucleotide exchange factor that regulates the recruitment of proteins to membranes by mediating GDP to GTP exchange. The encoded protein is localized to the Golgi apparatus and plays a role in vesicular trafficking by activating ADP ribosylation factor 1. The encoded protein has also been identified as an important host factor for viral replication. Multiple transcript variants have been observed for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human GBF1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, RatMouse (96%), Rat (96%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ARF1GEF antibody, FLJ21263 antibody, FLJ21500 antibody, KIAA0248 antibody, MGC134877 antibody, MGC134878 antibody, GBF1 antibody, Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 antibody, BFA-resistant GEF 1 antibody, golgi brefe |
|---|---|
| Molecular Weight(MW) | 206 kDa (note) (3) |
Application
Sample (30 ug of whole cell lysate) A: Hela 5% SDS PAGE GBF1 antibody diluted at 1:1000
Application
GBF1 antibody [N1], N-term detects GBF1 protein at cytoplasm on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. GBF1 antibody [N1], N-term dilution: 1:500.
Application
Immunofluorescence analysis of methanol-fixed HeLa, using GBF1 antibody at 1:200 dilution.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
