Application
Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with GBX2 antibody diluted at 1:1000.Application
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with GBX2 antibody diluted at 1:1000.Application
GBX2 antibody detects GBX2 protein by western blot analysis. IMR32 whole cell extracts and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GBX2 antibody diluted by 1:1000.Application
Rat tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with GBX2 antibody diluted at 1:1000.Application
GBX2 antibody detects GBX2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GBX2 antibody diluted by 1:1000.Application
GBX2 antibody detects GBX2 protein at nucleus by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GBX2 protein stained by GBX2 antibody diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:50. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Product Name | GBX2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GBX2 (gastrulation brain homeobox 2)May act as a transcription factor for cell pluripotency and differentiation in the embryo. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human GBX2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | BovineBovine (96%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | gastrulation brain homeobox 2 |
|---|---|
| Target | GBX2 |
| Molecular Weight(MW) | 37 kDa (note) |
| Cellular Localization | Nucleus |
Application
Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with GBX2 antibody diluted at 1:1000.
Application
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with GBX2 antibody diluted at 1:1000.
Application
GBX2 antibody detects GBX2 protein by western blot analysis. IMR32 whole cell extracts and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GBX2 antibody diluted by 1:1000.
Application
Rat tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with GBX2 antibody diluted at 1:1000.
Application
GBX2 antibody detects GBX2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GBX2 antibody diluted by 1:1000.
Application
GBX2 antibody detects GBX2 protein at nucleus by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GBX2 protein stained by GBX2 antibody diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:50. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
