Application
Sample (30 ug of whole cell lysate) A: A431 7.5% SDS PAGE diluted at 1:1000Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using GCC1 antibody at 1:500 dilution.Application
Immunofluorescence analysis of methanol-fixed A431, using GCC1 antibody at 1:200 dilution.| Product Name | GCC1 antibody [C1C3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GCC1 (GRIP and coiled-coil domain containing 1)The protein encoded by this gene is a peripheral membrane protein. It is sensitive to brefeldin A. This encoded protein contains a GRIP domain which is thought to be used in targeting. It may play a role in the organization of trans-Golgi network subcompartment involved with membrane transport. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human GCC1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rabbit, Rhesus Monkey, BovineMouse (93%), Rabbit (87%), Rhesus Monkey (97%), Bovine (88%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FLJ22035 antibody, GCC1P antibody, GCC88 antibody, MGC20706 antibody, GCC1 antibody, golgi coiled-coil 1 antibody, GRIP and coiled-coil domain-containing protein 1 antibody, golgi coiled-coil protein 1 antibody, peripheral membrane Golgi protein antibody, |
|---|---|
| Molecular Weight(MW) | 88 kDa (note) (2) |
| Cellular Localization | Cytoplasm , Golgi apparatus membrane; Peripheral membrane protein |
Application
Sample (30 ug of whole cell lysate) A: A431 7.5% SDS PAGE diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using GCC1 antibody at 1:500 dilution.
Application
Immunofluorescence analysis of methanol-fixed A431, using GCC1 antibody at 1:200 dilution.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
