Application
WB analysis of human fetal liver tissue using GCLC antibody at 0.2-1μg/ml.Application
IHC-P analysis of human bronchial epithelial tissue using GCLC antibody at 1:600. Left to right : DAPI, GCLC, Merge. Low pH, heat-induced antigen retrieval method utilizing Sodium Citrate buffer.Application
WB analysis of human liver tissue using GCLC antibody at 1:1000. Lane A : Primary antibody Lane B : Primary antibody + Blocking peptide| Product Name | GCLC antibody, N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GCLCGlutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.[provided by RefSeq, Oct 2010] |
| Immunogen | A synthetic peptide corresponding to a N-terminal region of Human GCLC |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 93%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 79% |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | GCLC , GCS , GCL , GLCLC , GLCL |
|---|---|
| Target | GCLC |
| Molecular Weight(MW) | 73 KDa (note) |
Application
WB analysis of human fetal liver tissue using GCLC antibody at 0.2-1μg/ml.
Application
IHC-P analysis of human bronchial epithelial tissue using GCLC antibody at 1:600. Left to right : DAPI, GCLC, Merge. Low pH, heat-induced antigen retrieval method utilizing Sodium Citrate buffer.
Application
WB analysis of human liver tissue using GCLC antibody at 1:1000. Lane A : Primary antibody Lane B : Primary antibody + Blocking peptide| Positive Control | human fetal liver , human liver , mouse heart |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. For short-term storage, store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
