Application
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using GCN2 antibody at 1:500 dilution.Application
Sample (30 µg of whole cell lysate) A: H1299 B: Molt-4 5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
GCN2 antibody [C2C3], C-term detects EIF2AK4 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: EIF2AK4 protein stained by GCN2 antibody [C2C3], C-term diluted at 1:500. Blue: Hoechst 33343 staining.| Product Name | GCN2 antibody [C2C3], C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GCN2 (eukaryotic translation initiation factor 2 alpha kinase 4)EIF2AK4 belongs to a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor-2 (EIF2S1; MIM 603907) to downregulate protein synthesis in response to varied cellular stresses (Berlanga et al., 1999 [PubMed 10504407]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human GCN2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, RatMouse (92%), Rat (92%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | GCN2 antibody, KIAA1338 antibody, EIF2AK4 antibody, GCN2-like protein antibody, eukaryotic translation initiation factor 2-alpha kinase 4 antibody, GCN2 eIF2alpha kinase antibody, eukaryotic translation initiation factor 2 alpha kinase 4 antibody |
|---|---|
| Molecular Weight(MW) | 187 kDa (note) |
Application
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using GCN2 antibody at 1:500 dilution.
Application
Sample (30 µg of whole cell lysate) A: H1299 B: Molt-4 5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
GCN2 antibody [C2C3], C-term detects EIF2AK4 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: EIF2AK4 protein stained by GCN2 antibody [C2C3], C-term diluted at 1:500. Blue: Hoechst 33343 staining.| Positive Control | H1299 , Molt-4 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
