Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using GCP2 antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: NIH-3T3 7.5% SDS PAGE diluted at 1:2000Application
Sample (30 ug of whole cell lysate) A: A431 B: Hela 7.5% SDS PAGE diluted at 1:1000Application
GCP2 antibody [N1N2], N-term detects TUBGCP2 protein at centrosome by confocal immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TUBGCP2 protein stained by GCP2 antibody [N1N2], N-term diluted at 1:500. Blue: Hoechst 33342 staining. [Images captured by Olympus FV10i Confocal Laser Scanning Microscope]| Product Name | GCP2 antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GCP2 (tubulin, gamma complex associated protein 2)Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome. |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human GCP2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, BovineRat (88%), Bovine (87%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | GCP2 antibody, MGC138162 antibody, SPBC97 antibody, Spc97p antibody, TUBGCP2 antibody, gamma-ring complex protein 103 kDa antibody, hGrip103 antibody, spindle pole body protein Spc97 homolog antibody, h103p antibody, GCP-2 antibody, gamma-tubulin complex p |
|---|---|
| Molecular Weight(MW) | 103 kDa (note) (2) |
| Cellular Localization | Centrosome |
Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using GCP2 antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: NIH-3T3 7.5% SDS PAGE diluted at 1:2000
Application
Sample (30 ug of whole cell lysate) A: A431 B: Hela 7.5% SDS PAGE diluted at 1:1000
Application
GCP2 antibody [N1N2], N-term detects TUBGCP2 protein at centrosome by confocal immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TUBGCP2 protein stained by GCP2 antibody [N1N2], N-term diluted at 1:500. Blue: Hoechst 33342 staining. [Images captured by Olympus FV10i Confocal Laser Scanning Microscope]| Positive Control | A431 , HeLa , NIH-3T3 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
