| Product Name | G-CSF antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to G-CSFGranulocyte Colony Stimulating Factor(G-CSF) is a potent stimulator of bone marrow cells especially those of neutrophil lineage. |
| Immunogen | Highly pure (>98%) recombinant mG-CSF (mouse Granulocyte Colony Stimulating Factor ). |
| Clonality | Polyclonal |
|---|---|
| Isotype | Unknown |
| Host Species | Rabbit |
| Tested Applications | ELISANeutWB |
| Neutralization:To yield one-half maximal inhibition [ND 50 ] of the biological activity of mG-CSF (0.5 ng/ml), a concentration of 0.005 - 0.010 μg/ml of this antibody is required. ELISA:To detect mG-CSF by direct ELISA (using 100μl/well antibody solution) a concentration of at least 0.5μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mG-CSF. Western Blot:To detect mG-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mG-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. | |
| Species Reactivity | Mouse |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Csf3, G-CSF, 12985, Csfg, GSF3, GCSF, G CSF |
|---|---|
| Target | G-CSF |
| Application Notes | Neutralization:To yield one-half maximal inhibition [ND 50 ] of the biological activity of mG-CSF (0.5 ng/ml), a concentration of 0.005 - 0.010 μg/ml of this antibody is required. ELISA:To detect mG-CSF by direct ELISA (using 100μl/well antibody solution) a concentration of at least 0.5μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mG-CSF. Western Blot:To detect mG-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mG-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | PBS, pH 7.4, no preservative, sterile filtered |
