Application
IHC-P analysis of human angiosarcoma using G-CSF antibody at a concentration of 0.25 μg/ml. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended.| Product Name | G-CSF antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to G-CSFThe protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010] |
| Immunogen | The immunogen corresponding to a region within amino acids 31 and 207 of human G-CSF. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PNeutWB |
| We recommend the following starting dilutions: For WB: Use at a concentration of 0.1-0.2 µg/ml. For ELISA: Use at a concentration of 0.5 - 2.0 μg/ml. For IHC-P: Use at a concentration of 0.25 μg/mL. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. For Neutralization: one-half maximal inhibition [ND50] of the biological activity of hG-CSF (0.5 ng/ml), a concentration of 0.04-0.06 µg/ml of this antibody is required. Optimal dilutions should be determined experimentally by the end user. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CSF3 , colony stimulating factor 3 (granulocyte) , C17orf33 , CSF3OS , GCSF |
|---|---|
| Target | G-CSF |
Application
IHC-P analysis of human angiosarcoma using G-CSF antibody at a concentration of 0.25 μg/ml. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended.| Application Notes | We recommend the following starting dilutions: For WB: Use at a concentration of 0.1-0.2 µg/ml. For ELISA: Use at a concentration of 0.5 - 2.0 μg/ml. For IHC-P: Use at a concentration of 0.25 μg/mL. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. For Neutralization: one-half maximal inhibition [ND50] of the biological activity of hG-CSF (0.5 ng/ml), a concentration of 0.04-0.06 µg/ml of this antibody is required. Optimal dilutions should be determined experimentally by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS, pH 7.2 |
