Application
Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC 7.5% SDS PAGE diluted at 1:1000Application
Sample (30 ug of whole cell lysate) A: 293T B: A431 C: H1299 7.5% SDS PAGE diluted at 1:500Application
GDF5 antibody [C3], C-term detects GDF5 protein at cytosol on human breast cancer by immunohistochemical analysis. Sample: Paraffin-embedded breast cancer. GDF5 antibody [C3], C-term dilution: 1:250.| Product Name | GDF5 antibody [C3], C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GDF5 (growth differentiation factor 5)The protein encoded by this gene is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Mutations in this gene are associated with acromesomelic dysplasia, Hunter-Thompson type; brachydactyly, type C; and chondrodysplasia, Grebe type. These associations confirm that the gene product plays a role in skeletal development. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human GDF5. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-P,WB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human,Mouse |
| Predicted Reactivity | Xenopus laevis, Pig, Rabbit, Chicken, BovineXenopus laevis (100%), Pig (100%), Rabbit (100%), Chicken (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | BMP14 antibody, CDMP1 antibody, LAP4 antibody, OS5 antibody, SYNS2 antibody, GDF5 antibody, cartilage-derived morphogenetic protein 1 antibody, growth/differentiation factor 5 antibody, GDF-5 antibody, cartilage-derived morphogenetic protein-1 antibody, ra |
|---|---|
| Molecular Weight(MW) | 55 kDa (note) (2) |
| Cellular Localization | Secreted |
Application
Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC 7.5% SDS PAGE diluted at 1:1000
Application
Sample (30 ug of whole cell lysate) A: 293T B: A431 C: H1299 7.5% SDS PAGE diluted at 1:500
Application
GDF5 antibody [C3], C-term detects GDF5 protein at cytosol on human breast cancer by immunohistochemical analysis. Sample: Paraffin-embedded breast cancer. GDF5 antibody [C3], C-term dilution: 1:250.| Positive Control | 293T , A431 , H1299 , NIH-3T3 , JC |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
