Application
GEF-H1 antibody detects GEF-H1 protein by western blot analysis. A. 30 µg Raw264.7 whole cell lysate/extract B. 30 µg C2C12 whole cell lysate/extract 5% SDS-PAGE GEF-H1 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (30 µg of whole cell lysate) A: 293T B: A431 C: HeLa 5% SDS PAGE diluted at 1:2000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (10 µg of whole cell lysate) A: hMSC-3A6 B: siRNA of ARHGEF2/GEF-H1 8% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunoprecipitation of GEF-H1 protein from 293T whole cell extracts using 5 μg of GEF-H1 antibody . Western blot analysis was performed using GEF-H1 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
GEF-H1 antibody detects GEF-H1 protein by western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg GL261 whole cell lysate/extract C. 30 µg C8D30 whole cell lysate/extract D. 30 µg NIH-3T3 whole cell lysate/extract E. 30 µg BCL-1 whole cell lysate/extract F. 30 µg Raw 264.7 whole cell lysate/extract G. 30 µg C2Cl2 whole cell lysate/extract 5% SDS-PAGE GEF-H1 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
GEF-H1 antibody detects GEF-H1 protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HeLa whole cell lysate/extract D. 30 µg HepG2 whole cell lysate/extract 5% SDS-PAGE GEF-H1 antibody dilution: 1:2000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
GEF-H1 antibody detects GEF-H1 protein at cytosol on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. GEF-H1 antibody dilution: 1:500.Application
GEF-H1 antibody detects GEF-H1 protein at cytosol on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. GEF-H1 antibody dilution: 1:500.Application
GEF-H1 antibody detects ARHGEF2 protein at Cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in -20℃ 100% MeOH for 5 min. Green: ARHGEF2 protein stained by GEF-H1 antibody diluted at 1:500. Blue: Hoechst 33343 staining.Application
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using GEF-H1 antibody at 1:500 dilution.| Product Name | GEF-H1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GEF-H1 (Rho/Rac guanine nucleotide exchange factor (GEF) 2)Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate rho-dependent signals. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human GEF-H1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Dog, Pig, BovineDog (92%), Pig (96%), Bovine (94%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp547L106 antibody, DKFZp547P1516 antibody, FLJ45440 antibody, GEF antibody, GEF-H1 antibody, GEFH1 antibody, KIAA0651 antibody, LFP40 antibody, P40 antibody, ARHGEF2 antibody, microtubule-regulated Rho-GEF antibody, Rho/Rac guanine nucleotide exchange |
|---|---|
| Molecular Weight(MW) | 112 kDa (note) (3) |
| Cellular Localization | Cytoplasm , Cell junction , tight junction , Golgi apparatus , Spindle |
Application
GEF-H1 antibody detects GEF-H1 protein by western blot analysis. A. 30 µg Raw264.7 whole cell lysate/extract B. 30 µg C2C12 whole cell lysate/extract 5% SDS-PAGE GEF-H1 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (30 µg of whole cell lysate) A: 293T B: A431 C: HeLa 5% SDS PAGE diluted at 1:2000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (10 µg of whole cell lysate) A: hMSC-3A6 B: siRNA of ARHGEF2/GEF-H1 8% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunoprecipitation of GEF-H1 protein from 293T whole cell extracts using 5 μg of GEF-H1 antibody . Western blot analysis was performed using GEF-H1 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
GEF-H1 antibody detects GEF-H1 protein by western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg GL261 whole cell lysate/extract C. 30 µg C8D30 whole cell lysate/extract D. 30 µg NIH-3T3 whole cell lysate/extract E. 30 µg BCL-1 whole cell lysate/extract F. 30 µg Raw 264.7 whole cell lysate/extract G. 30 µg C2Cl2 whole cell lysate/extract 5% SDS-PAGE GEF-H1 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
GEF-H1 antibody detects GEF-H1 protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HeLa whole cell lysate/extract D. 30 µg HepG2 whole cell lysate/extract 5% SDS-PAGE GEF-H1 antibody dilution: 1:2000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
GEF-H1 antibody detects GEF-H1 protein at cytosol on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. GEF-H1 antibody dilution: 1:500.
Application
GEF-H1 antibody detects GEF-H1 protein at cytosol on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. GEF-H1 antibody dilution: 1:500.
Application
GEF-H1 antibody detects ARHGEF2 protein at Cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in -20℃ 100% MeOH for 5 min. Green: ARHGEF2 protein stained by GEF-H1 antibody diluted at 1:500. Blue: Hoechst 33343 staining.
Application
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using GEF-H1 antibody at 1:500 dilution.| Positive Control | 293T , A431 , BCL-1 , C2C12 , C8D30 , GL261 , HeLa , HepG2 , NIH-3T3 , Neuro 2A , Raw264.7 , hMSC-3A6 , *mouse fibroblast |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
